Akriti Baby Kanth

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Probable reasons for inaccurate activation of naive CD4+ T cells after negative MACS selection technique ?

Can anyone point out the reasons for inaccurate activation of nave T cells post negative MACS selection ? I had pre-coated wells of 48 well plate with 2ug/mL of antiCD3 abs and had it incubated...

12 February 2018 8,315 0 View

How can I improve the purity of RNA sample extracted from a low cell count, say 1 * 10^5 cells/mL ?

I am a beginner at this. I have recently begun isolating RNAs from differentiated T cells and my efficiency has been the worst! I really don't intend to waste any reagents or sacrifice many more...

19 December 2017 9,193 4 View