Akram Taherinasab

3 Questions 2 Answers 0 Followers

Questions related from Akram Taherinasab

Has anyone tried to transfect sup-b15 cell line using recombinant cas9?

Hello everyone, I am starting a project in which I'm planning to create a cell line with a specific deletion. I am going to use SUP-B15 and I need to know if anyone has ever transfected this cell...

07 July 2018 4,183 1 View

In vitro transcription of S. pyogenes Cas9 sgRNAs from two complementary oligos??

Hi, Has anyone tried to synthesize the sgRNA without using a kit? Can anyone help me with the protocol? I found a Protocol on Benchling blog but I am not sure if it is going to work. In this...

04 April 2018 9,480 2 View

How to do Knock in using CRISPR/Cas9 through Electroporation system??

Hello, I am trying to study two mutations in a multi exonic gene. My gene is TP53 with 11 exones and I have Raji Cell line which has 2 mutation : one in Exon 6 and the other one in exone 7. For...

02 February 2018 9,386 3 View