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When performing Gateway vector cloning and transforming Agrobacterium cells for plant transformation or transient expression of a gene of interest in tobacco leaves: Is it necessary to generate...
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I am expressing several genes using the pET28(a) vector in E. coli BL21 cells. While some recombinant proteins are expressed, they accumulate predominantly in inclusion bodies. Although I purify...
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I am trying to clone a few genes into the pENTR/D-TOPO vector. To do this, I use a previously cloned plasmid as a template for PCR product amplification. The PCR product is successfully amplified,...
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