Akansha Sharma

2 Questions 2 Answers 0 Followers

Questions related from Akansha Sharma

Is there any commercially available affinity column for native purification of cysteine protease?

Hi all, I am planning to purify native cysteine protease from a plant source but not able to find any affinity column for the protein. Does anyone has some idea or have used in his/her work then...

27 December 2016 9,396 3 View

NOT ABLE TO CLONE MY GENE INTO pET28a+ VECTOR. CAN ANYONE HELP!!

I am trying to clone a chemically synthesised gene (1092 bp) into a expression vector called pET28a+ vector (5.3 kb). After PCR amplification of my gene of interest from the chemically synthesised...

26 March 2016 9,707 3 View