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I am trying to express a recombinant protein which is a cysteine protease in E.coli expression system since a very long time but it is not expressing efficiently as it should be. I have used...
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Hi all, I am planning to purify native cysteine protease from a plant source but not able to find any affinity column for the protein. Does anyone has some idea or have used in his/her work then...
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I am trying to clone a chemically synthesised gene (1092 bp) into a expression vector called pET28a+ vector (5.3 kb). After PCR amplification of my gene of interest from the chemically synthesised...
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