Akansha Madhawan

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Questions related from Akansha Madhawan

Can anyone help me in cloning miRNA in pCAMBIA1302 vector?

I want to clone miRNA (pre-miRNA) of 250bp. Firstly I cloned in pJET 1.2 vector with NcoI and BglII restriction site added in pre-miRNA sequence. Cloning in pJET1.2 vector is confirmed by...

15 September 2021 3,051 2 View

Can anyone suggest me in vivo assay for capturing long non coding RNA and its interacting partner miRNA?

On the basis of in-silico analysis we hypothesized that a long non-coding (lncRNAs) interacts with miRNA thus inhibiting that miRNA function in bread wheat. Now I want to isolate this lncRNA-miRNA...

13 August 2020 7,709 2 View