Is it advisable to seed A549 cells in less number in a plate and start the treatment 48 hours after seeding till they become full confluent?

I do inflammatory cell signalling related experiments. I seed A549 cell in a 6-well plate (for eg.) and wait for 48 hours to get them confluent. Then serum starvation overnight, followed by...

27 January 2016 5,746 2 View