Ajmeri Sultana Shimu

2 Questions 5 Answers 0 Followers

Questions related from Ajmeri Sultana Shimu

How to improve the lentivirus transduction efficiency into MC38 cells?

Hi, I am introducing the strategy briefly which I follow to infect the MC38 cells with harvested lentivirus media from HEK293T cells at 24 and 48h. 1. First, MC38 cells are cultured in 60mm...

25 July 2023 1,412 6 View

What is the problem to cause cell death and low ratio of Th17 T cells after induction of CD4+T cells into Th17 cells in vitro?

First, we coated 96 well TC-treated plates with 5ug/ml anti-CD3 antibody (17A2, biolegend) Then, we added Naive CD4+ CD25- CD45RB high T cells (500000) in each well after sorting through flow...

01 April 2023 500 2 View