Ajinkya Patil

4 Questions 7 Answers 0 Followers

Questions related from Ajinkya Patil

Is there a protein that can induce apoptosis in cancer cells?

I am designing a CRISPR based assay and need help designing a positive control. Specifically, can someone tell me a protein which causes cell death when it is expressed in cancer cells ? I prefer...

10 August 2015 5,644 5 View

Is there a protein that can induce apoptosis in cancer cells?

I am designing a CRISPR based assay and need help designing a positive control. Specifically, can someone tell me a protein which causes cell death when it is expressed in cancer cells ? I prefer...

08 August 2015 1,278 5 View

How can I avoid QPCR NTC contamination?

I am running qPCR assays for porcine parvo virus. The assay is running fine except for the fact that my negative control wells show an amplification curve every time I run the assay. The Ct values...

09 September 2014 1,986 12 View

Why does SYBR Gold interfere with 1Kb+ DNA ladder?

I have recently shifted from EtBr to SYBR GOLD for staining my DNA in agarose gels. But the 1kb+ DNA ladder in the SYBR GOLD gels are melting, rendering them useless. I am assuming this has...

04 August 2014 7,820 4 View