Ajinkya sunil Rao

3 Questions 5 Answers 0 Followers

Questions related from Ajinkya sunil Rao

How to verify whether MANT-GDP bound to Ras protein or not?

I am doing nucleotide exchange assay using Tecan spark 10 M machine.I have loaded KRas with MANT-GDP and used 1uM of the same and the relative Fluorescence units is around 32000. But the...

31 March 2019 6,115 4 View

Does anyone know how to load GppNhp to Ras protein ? I am trying to load GppNHp (non-hydrolysable GTP) on RAS for my GST pull down and is not working?

Please provide the reference paper if you know? I am using wt RAS and want to just label it with GTP. Do we need to change buffers for GTP loading? I have currently isolated RAS using Ni2+-NTA...

24 April 2018 1,318 3 View

How to prepare E.Coli cell lysate as I want to use it as negative control during SDS PAGE alongside my protein lysate ?

I tried using E.coli BL21 cells -grew them in 2TY media for about 2 hours ,centrifuged and lysed the cells using lysozyme and DNAse ? But i only got a single band at the bottom of my SDS Gel .

19 August 2017 6,498 3 View