Ajinkya sunil Rao

2 Questions 4 Answers 0 Followers

Questions related from Ajinkya sunil Rao

How to verify whether MANT-GDP bound to Ras protein or not?

I am doing nucleotide exchange assay using Tecan spark 10 M machine.I have loaded KRas with MANT-GDP and used 1uM of the same and the relative Fluorescence units is around 32000. But the...

31 March 2019 5,960 4 View

How to prepare E.Coli cell lysate as I want to use it as negative control during SDS PAGE alongside my protein lysate ?

I tried using E.coli BL21 cells -grew them in 2TY media for about 2 hours ,centrifuged and lysed the cells using lysozyme and DNAse ? But i only got a single band at the bottom of my SDS Gel .

19 August 2017 6,354 3 View