Why PCR bands are missing when gel check after DNA isolation(using Nucleospin Insect Kit) revealed bands- Gene is insect COI, primers universal?

I am working on Molecular identification (gene COI) of parasitoids (Chalcid Wasps). After DNA isolation by using Nucleospin kit, Gel check confirmed bands for most of the samples. But, PCR bands...

10 October 2018 8,540 3 View