Ajaya Karkee

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Questions related from Ajaya Karkee

How can i decide the best annealing temperature of the primers (Gel electrophoresis photographs is attached here)?

I used four primers (Primer 2,4, 5 and 6) with 2 rice variety and tested its annealing temperature at 53, 54, 55, 56,57 and 58 degree centigrade on PCR. The photograph of the gel electrophoresis...

09 September 2019 7,120 5 View

For calculations of Mann-Kendall and Sen’s Slope, MS-Excel program called MAKESENS version 1.0 developed by FMI is suitable?

For the calculation of climate change trends by Mann-Kendall and Sen's slope, MS-Excel program called MAKESENS version 1.0 developed by Finnish Meteorological Institute is downloaded from...

09 September 2019 9,497 4 View

What is the annealing temperatures of RM 166, RM 155 and RM 5647 primers?

These primers are SSR markers for blast resistant genes of Rice.

08 August 2019 5,397 5 View