Aisha Khodijah Kholib Jati

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Questions related from Aisha Khodijah Kholib Jati

Why my seropositive ELISA samples appear negative in PCR?

I'm doing prevalence study of Toxoplasmosis using both serology (ELISA, Platelia Toxo IgG & Platelia Toxo IgM kit, BioRad USA) and molecular method (PCR). During ELISA assay, my samples were...

11 November 2018 4,702 12 View

Why my internal control band did not appear with same intensity even I have used the same concentration, same volume and also PCR cycling conditions?

I'm currently doing PCR for my study. I used heterologous internal control which I need to include plasmid (Vibrio Cholerae HemM Gene) in each samples. But after I've run about 2 to 3 times pcr,...

11 November 2018 9,516 7 View