Aisha Khodijah Kholib Jati

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Questions related from Aisha Khodijah Kholib Jati

How to optimize and maximize extracted DNA yield from human stool samples ?

How to optimize and maximize extracted DNA yield from human stool samples? The stool samples will be collected without using any preservation liquid and might be freeze and extracted the next...

08 June 2021 7,550 1 View

Is it okay if we do not include "internal control" in our PCR?

Based on paper I read, internal controls (ICs) constructed to provide assurance that clinical specimens are successfully amplified and detected. But some paper did not mentioned any ICs they used...

13 January 2019 1,052 1 View

Why my seropositive ELISA samples appear negative in PCR?

I'm doing prevalence study of Toxoplasmosis using both serology (ELISA, Platelia Toxo IgG & Platelia Toxo IgM kit, BioRad USA) and molecular method (PCR). During ELISA assay, my samples were...

08 November 2018 8,089 10 View

Why my internal control band did not appear with same intensity even I have used the same concentration, same volume and also PCR cycling conditions?

I'm currently doing PCR for my study. I used heterologous internal control which I need to include plasmid (Vibrio Cholerae HemM Gene) in each samples. But after I've run about 2 to 3 times pcr,...

07 November 2018 7,023 6 View