Aimilia Lydia Kalafateli

4 Questions 5 Answers 0 Followers

Questions related from Aimilia Lydia Kalafateli

Experience with EnCorBio antibodies for IF?

Hi everyone, I'll be performing IF staining in free floating perfused rat brain slices. I'm interested in Tau and MAP2 as neuronal markers and I found this company EnCorBio to have very nice...

26 April 2019 8,590 0 View

How could I possibly investigate if a hormone crosses the BBB?

Dear all, I am currently working with ghrelin (a gut peptide) and I would like to check if the peptide crosses the blood brain barrier and if it is possible to find out in which areas it reaches....

10 March 2016 7,256 5 View

CDNA synthesis: how can I dilute low RNA concentration of 66 ng/ul for a 1ug reaction?

Hello everyone, I just completed RNA extraction from brain tissue (PFC) using the RNeasy Lipid Mini kit from QIAGEN. One particular sample gave a very low concentration of 66 ng/ul RNA (in a total...

01 December 2015 605 7 View

Which is the best antibody for detection of phospho-p70S6K used in Western Blots?

I am doing some Western Blots and I want to trace bands of the phosphorylated p70S6K in mice brain tissue. I have repeated all my experiments more than 4 times and I still get no or very low...

11 August 2013 3,626 7 View