Aimilia Lydia Kalafateli

3 Questions 5 Answers 0 Followers

Questions related from Aimilia Lydia Kalafateli

How could I possibly investigate if a hormone crosses the BBB?

Dear all, I am currently working with ghrelin (a gut peptide) and I would like to check if the peptide crosses the blood brain barrier and if it is possible to find out in which areas it reaches....

10 March 2016 7,082 5 View

CDNA synthesis: how can I dilute low RNA concentration of 66 ng/ul for a 1ug reaction?

Hello everyone, I just completed RNA extraction from brain tissue (PFC) using the RNeasy Lipid Mini kit from QIAGEN. One particular sample gave a very low concentration of 66 ng/ul RNA (in a total...

01 December 2015 558 7 View

Which is the best antibody for detection of phospho-p70S6K used in Western Blots?

I am doing some Western Blots and I want to trace bands of the phosphorylated p70S6K in mice brain tissue. I have repeated all my experiments more than 4 times and I still get no or very low...

11 August 2013 3,559 7 View