Sudden death of neural cell cultures following DIV15 media change - why?

Hi, I am culturing cortical neural cultures derived from P0-1 rats. Cells are plated onto poly-D-lysine coated dishes and survive nicely in NeurobasalA + B27 + GlutaMax + PenStrep - 50% of the...

30 July 2019 3,394 3 View