Aikaterini Papagianni

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Questions related from Aikaterini Papagianni

Any suggestions for anti-HA antibodies raised in rat or goat for mouse tissue immunostaining?

Any suggestions for anti-HA antibodies raised in rat or goat for mouse tissue immunostaining? Looking to co-stain with antibodies raised in rabbit.

19 May 2021 2,890 2 View

Is a lysis buffer containing NP-40 enough to extract nuclear proteins?

I am trying to extract a nuclear protein of 0-3h Drosophila melanogaster embryos. My lysis buffer is composed of NP-40, Tris pH 8, NaCl and a cocktail of protease inhibitors. Is this enough to...

18 October 2013 726 2 View