Aidan Kenny

3 Questions 3 Answers 0 Followers

Questions related from Aidan Kenny

How much of an mRNA UTR to include as an input into mFold?

The mFold input has a limit of 2400nt and while searching for common secondary strucutres around miRNA binding site of mRNA we have come across the issue of mRNAs with extremely long 3' UTRs, up...

28 June 2021 7,973 3 View

Taqman microRNA Assay Primer dimerisation at exceptionally high levels?

I have been using taqman microRNA assays for 3 years and have recently come across a very serious problem with one particular microRNA. RT-qPCR analysis shows the miRNA to be expressed at higher...

23 November 2018 8,268 3 View

Does the populations of argonaute-2 microRNAs and RISC loaded miRNA correlate with total miRNA within cells/tissue?

We've performed Profiling of Ago-2 RIP miRNA in tissue and also followed it with individual RT-qPCRs and showed that the miRNA levels between the 2 were correlated but this is with a handul of...

03 February 2018 1,936 2 View