Aicha Kriaa

2 Questions 3 Answers 0 Followers

Questions related from Aicha Kriaa

Shaving Gram-positive bacteria ?

I'm looking for a detailed protocol in order to selectively digest bacterial surface proteins, but I'm not sure whether I'll use trypsin or proteinase K ? Many thanks.

18 July 2016 671 1 View

How can I over express an active protease in E.coli BL21 (DE3)?

I'm trying to over express an active protease in BL21 (DE3) following the setup below: 1. Inoculate starter culture at a 1:100 dilution into LB broth2. Incubate at 37°C with shaking until OD600...

01 April 2016 5,641 5 View