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Questions related from Ahmet Alptekin
Hi, I am doing insertion mutagenesis. I am using Q5 from NEB and optimized all the steps. At the end, I am getting ~5-30 colonies. In one case, I am trying insertion mutagenesis. After PCR, KLD...
29 March 2021 4,149 3 View
Hi. I am trying site directed mutagenesis (deletion and insertion together in the same site) but so far couldn't get colonies. My plasmid is ~13kb and I will delete ~30bp and insert similar size...
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I'm having trouble with gel purification. After restriction digestion of PCR product for cloning, I am running DNA on a gel to purify . Yield is so low 5-10%. (Normally, yield is ~30% in my hand...
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Through UCSC genome browser, I can see which transcription factor binds to regulator site of my gene ( Transcription factors, which have been ChIP-sequenced so far ). I have a list of...
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