Ahmet Alptekin

3 Questions 8 Answers 0 Followers

Questions related from Ahmet Alptekin

Insertion mutagenesis, missing T base corresponds to first base of forward primer, any thoughts?

Hi, I am doing insertion mutagenesis. I am using Q5 from NEB and optimized all the steps. At the end, I am getting ~5-30 colonies. In one case, I am trying insertion mutagenesis. After PCR, KLD...

29 March 2021 4,138 3 View

Site directed mutagenesis, successful PCR but no colonies. Suggestions?

Hi. I am trying site directed mutagenesis (deletion and insertion together in the same site) but so far couldn't get colonies. My plasmid is ~13kb and I will delete ~30bp and insert similar size...

24 November 2020 6,604 8 View

DNA gel purification problem

I'm having trouble with gel purification. After restriction digestion of PCR product for cloning, I am running DNA on a gel to purify . Yield is so low 5-10%. (Normally, yield is ~30% in my hand...

25 May 2017 3,258 8 View