Ahmed Hassan

4 Questions 1 Answers 0 Followers

Questions related from Ahmed Hassan

His tagged protein expression in mammalian cell?

what is the best kit available for purifying proteins with his-tag expressed in 293 cells??

05 May 2016 9,275 4 View

Animal model for Zika vaccine?

Is there any animal model established for testing zika vaccines?? NHP/mice? Any reference regarding that please?

03 March 2016 8,216 3 View

Can I perform ELISpot for splenocytes that have been kept at 4C for few days (4 -5 days)?

I got the splenocytes from mice spleens and I did ELISpot for the first time. Unfortunately, something had gone wrong and I didn't get the spots. I stored the remaining splenocytes at 4C. Can I...

01 January 2016 4,899 5 View

Is someone familiar with propagation of influenza H5N2 in embryonated chicken eggs?

I was trying to grow H5N2 virus through allantoic inoculation. I have tried several dilutions based on HA titer of the inoculum I have. I harvest the eggs 48 hours after inoculation and I never...

09 September 2014 6,167 4 View