Ahmed Shah

7 Questions 4 Answers 0 Followers

Questions related from Ahmed Shah

Can anyone suggest me spectrometeric analysis of Bacterial glycerate Kinase?

I want to know activity of glycerate kinase from my microbial culture. I am not able to find out suitable method to do it properly. Please suggest me better protocol for analysis of this enzyme.

06 October 2015 9,512 3 View

How can I chemically dye plastic stones for fabric Use?

I want to dye different shaped plastic stones chemically. Please tell me which kind of chemicals and techniques I can use to dye these stones for Fabric use.

30 December 2014 5,824 0 View

Which vector and strain of Bacillus sp. is most suitable for large scale fermentation of enzymes & proteins?

I want to produce proteins at large scale fermenter level in Bacillus sp..Please anyone tell me which strain and vector I should use for large scale fermentation of industrially important enzymes...

25 July 2014 4,525 1 View

Can anyone help with overproduction of invertase enzyme in fermenter?

I have cloned my Saccharomyces cerevisiae invertase gene in pichia pastoris X-33 strain using PpicZalpha vector. In large scale production it’s giving only 3000 U/ml titre, which is too low than...

15 April 2014 7,478 3 View

Any advice on a problem in production of Pectin Lyase D (1122bp) from A. niger in Pichia pastoris?

Dear all, I want to express pectin lyase D gene 1122bp (A. niger) in pichia pastoris X-33 using PpicZalpha A vector. In silico analysis showing translation of whole sequence in to amino acids,...

18 March 2014 9,231 2 View

Can anyone help with a problem in production of beta galactosidase(3021bp) from A. niger in pichia pastoris?

I am using beta galactosidase gene (3021bp) from A. niger to overproduce in Pichia pastoris. My vector is PpicZalphaA (3.6 kb). In spite of getting clone I am not getting expression in pichia...

03 January 2014 7,586 2 View

Any advice on a problem in production of beta galactosidase gene (3021bp) from A. niger in Pichia pastoris?

I am using beta galactosidase gene (3021bp) from A. niger to overproduce in Pichia pastoris. My vector is PpicZalphaA (3.6 kb). In spite of getting a clone I am not getting an expression in Pichia...

03 January 2014 257 4 View