How can I calculate the enzyme activity of LDH by using the reduction in NADH at 340nm?

I'm using 100 mM phosphate buffer (7.5), NADH final concentration of 0.13 mM. and sodium pyruvate in phosphate buffer to a final concentration of 2.3 mM. the ratio as the following: 933.3 ul...

09 May 2013 4,835 4 View