7 Questions 4 Answers 0 Followers
Questions related from Ahmed Mohamed Raslan
Hello, "I am observing an unusual phenomenon where the protein concentration in my flow-through sample is consistently higher than that of the lysate sample when run on gel, Still, I am...
13 April 2023 3,865 1 View
Hello, I need suggestions of databases, websites or review articles that have number of proteins of unknown structure. Can you guys recommend me any of these approaches so as to identify a number...
20 January 2023 7,768 0 View
Hello, I got a question about optimizing my FPLC separation for my 55 kd protein from a pre-attached solubility tag of 30 kd protein. I am using 6x his tag and tev cleavage. After doing the...
22 December 2022 6,266 3 View
Hello, I have a stock DNase solution of 2500 U/ml, it is supplied as 50% glycerol stocks in 10mM Tris-HCl pH 7.5, 10mM CaCl2, 10mM MgCl2. I need to know how much of this solution to add to about...
21 November 2022 8,372 0 View
Hello, Recently I had an issue with polymerization of SDS page. I used to add 5 ul TEMED and 50 ul of 10% APS for each 12 ml of SDS page but suddenly my SDS page stopped polymerizing and could...
09 November 2022 8,145 3 View
"BL21 (DE3)-RIL cells, which are compatible with the T7 promoter system, contain plasmids that encode arginine, isoleucine, and leucine tRNAs that are rare in E. coli, and are deficient in both...
15 June 2022 9,357 3 View
what is the role played by these dialysis buffer reagents ( MOPSO, DTT, glycerol) in TEV protease for purification of His-tagged proteins? also what is the dialysis bag used for?
09 June 2022 8,928 3 View
