Ahmed M. Kamel

4 Questions 6 Answers 0 Followers

Questions related from Ahmed M. Kamel

Can mineral (bottled) water be used instead of distilled water for gel electrophoresis?

I have a question that may sound silly. Can meniral water (and by that I mean commercial water purchased with the purpose of drinking) instead of distilled water that is usually used for the...

22 September 2016 2,554 14 View

Can I reuse a DNA extraction spin column?

I was using the promega DNA extraction kit to extract my DNA from whole blood and I used to resuspend DNA in RNAse free water and the PCR was working fine. Lately I used the genejet DNA extraction...

12 March 2015 6,153 9 View

Can I use a DNA purification kit to isolate the Product (because my PCR band is weak) and then re-amplify it?

I cant get new Dna and my band is weak.i need to digest the product.i heard purification kits do good job and I need the product just for conventional PCR so that I can increase yield to be able...

21 November 2014 6,358 9 View

How many ul of DNA template should be used for 50 ul PCR ?

I use promega DNA extraction kit. I use 300 ul of blood which should yield 5-15 ug genomic DNA .I dissolve DNA in 20 ul wfi and use 1 ul of this soln for PCR. Also should I leave DNA after I add...

10 October 2014 3,218 22 View