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Dear all, In my next experiment i need to clone a large fragment(7kbp) of DNA into a (3kbp) backbone using Gibson assembly kit. NEB suggests using Q5 High-Fidelity DNA polymerase for this kind of...
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Hey Guys, I usually use ultracentrifugation in the last step in Lentiviral production and I was wondering if I could use the FLP chromatography instead of the regular method ? which offers higher...
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Hello there guys, I am cloning into a lentiviral vector but unfortunate I am not getting any colonies. I restrict the CD55 (50 nanograms of DNA) gene using both AgeI and XhoI and I restrict the...
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