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Questions related from Ahmad Jawad Sabir
I am trying to concentrate a 43kDa soluble protein expressed in mammalian cells. I tried to concentrate it but the sample went through the column very quickly even at centrifugation speed lower...
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I have been doing affinity purification for a few months now, everything was working fine before but I am getting no protein with the newly packed columns. Antibody coupling efficiency is above...
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I am trying to hydrate CNBr activated Sepharose 4B using 1mM HCl, the resin was expired 10 years ago and it was purchased from Amersham Bioscience, Sweden, I am trying to get catalogue and details...
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I have done DNA extractions with a good quality kit and have got a fair concentration of DNA but its purity is really very low as A260/280 ratio is 1.45. Is there any way to get rid of...
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I am trying to purify a Flag-tagged recombinant protein expressed in mammalian cells. I had packed a couple of columns for this purpose using sepharose resin and it worked fine before. Now I have...
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