Ahmad Usman

10 Questions 17 Answers 0 Followers

Questions related from Ahmad Usman

How to measure (or predict) health of a DC contactor or a DC relay ?

I need help in understanding the mechanism to measure/estimate health of DC contactors and DC relays used in industrial grade power systems. What are the mechanisms and tools used for this purpose...

11 May 2020 1,578 3 View

Sodium periodate oxidation of antibodies ?

I am preparing a modified lectin based immuno assay. I am oxidizing my monoclonal anti-PSA antibodies (Abs) to avoid non-specific lectin attachment to the carbohydrated present of the Abs. I am...

26 February 2018 6,395 1 View

How to quantify capture efficiency of antigens in a microfluidic immunoassay ?

How can one quantify capture efficiency of antigens in a microfluidic immunoassay chip ? For example, if we have a anti-IgG coated microfluidic chip and we want to know the capture efficiency of...

18 October 2017 1,670 6 View

Protocols for diluting SU-8 for inkjet printing ?

I have SU-8 2000.5 and SU8- 2002 (from Microchem). I have to develop a protocol to decrease the viscosity for inkjet printing using Microfab inkjet printer. There are a few options available for...

25 January 2017 1,431 3 View

"Mannose" binding of "lectins" over "avidin" surface ?

I have avidin coated silicon surface (Silicon - APTES - EDC/NHS/Avidin). I am looking forward to attach lectins of the surface using the mannose/glucose residue chains on the avidin instead of...

24 January 2017 112 0 View

Is it necessary to remove "Sodium Azide" from the anti-body solution ?

I am looking forward to conjugate PSA antibodies (Monoclonal, Mouse, IgG1 - A67-B/E3 ) available...

21 July 2016 5,696 6 View

How to conjugate "PSA anti-bodies" with "NHS - esters" ?

I am looking for conjugation mechanism to conjugate a "photo-cleavable (PC) Biotin - NHS ester" to "PSA anti-bodies". I need the Biotin part for the Avidin/Biotin bonding on the surface and...

26 June 2016 435 2 View

Issues with SU-8 adhesion on glass substrate. Any suggestion ?

I am using SU-8 2015 for my microfluidic channel structures. The substrate is 1mm thick glass wafer. Issue : SU-8 flows off the substrate during the developing process. The adhesion is poor...

08 June 2016 284 15 View

Can I conjugate Avidin on APTES coated surface without using Biotin?

I am looking to attach "Avidin" to APTES coated silicon surface without using biotin bonding. What I have in mind is to use "EDC or EDAC + NHS + avidin solution" and immerse the APTES coated...

20 January 2016 882 4 View

Looking for a soft copy of a book titled "Electric Power Generation, Transmission, and Distribution" by S.N.Singh"

I have a hard form of the book with me. I am looking to have a .pdf version of it. Please share any link where I can have it. Thank you

01 January 1970 5,462 20 View