Ahmad Nasiri

5 Questions 16 Answers 0 Followers

Questions related from Ahmad Nasiri

Why non target enzyme has been downregulated while a target gene has been successfully over expressed?

After a successful over-expression of a key enzyme in a target pathway, Surprisingly, we have been noticed that another key Enzyme that leads our target pathway into a non-target-branch has been...

13 June 2021 519 12 View

Plasmid transformation protocol into Agrobacterium rhizogenes.?

I want to insert my 14kb recombinant plasmid into A.Rhizogenous, what should I do during my transformation. I transformed my plasmid with freeze/thaw transformation method, but it didn't work. I'm...

04 December 2019 1,101 7 View

Why I cant get transgenic hairy root while I get hairy roots already from native A. Rhizogenous?

I can get good hairy roots by Agrobacterium rhizogenous, but when I infect explants by A.rhizogenous that contain recombinant plasmid, I couldn’t get any hairy root. How much is possible to not...

28 October 2019 4,681 7 View

What is the minimum distance needed between two RE sites? Can they follow each other directly?

Can i double digest with HindIII and BamHI enzymes that have just 10 nucleotides cut site distance in MCS of vector for insertion? How about sacI and EcoRI with 12 nucleotides cut site...

24 September 2018 1,179 4 View

Multiplex Primers and Probes Competition in Real-Time PCR

Hello Everyone I have been working on 14 different virus strains to be detected utilizing TaqMan Probes. Although I get good results in Singleplex assays with the almost same Ct for all samples,...

01 January 1970 6,007 6 View