12 Questions 39 Answers 0 Followers
Questions related from Ahasan Habib
Maximum-likelihood (ML) tree based on GTR, why we choose this GTR mode to construct a phylogenetic tree? Could anybody explain it with ref/article if there is any? I may find answer in Nei and...
03 April 2016 5,991 9 View
Hi all, I have length-weight data of fish. Before doing analysis, should do log transform for both length and weight data? I know and did long transform for length data to remove size-dependent...
22 September 2015 2,885 7 View
Hi, after aligning DNA sequences (generally I do it with BioEdit or MAFFT), what should I do with dot like "-----", should I just removed from both end and from middle? My amplified BP is 426 for...
05 April 2015 9,664 12 View
Hi, is there any software to trim off primer sequence from sequence (full length sequence). Is it possible to do so using BioEdit? or is there any software to find/place primer sequence and trim...
06 October 2014 971 2 View
I have been trying many times to perform Arlequin but following error message showing.. #[ERROR # 5] : haplotype already defined #[ERROR # 6] : unable to read sample data So where might be...
25 April 2014 3,809 5 View
I have been trying and did set data as well, but Arlequin shows error/warning message. I am using mtDNA D-loop marker.
19 March 2014 9,043 2 View
Also, how can you convert a fasta format file to rdf? Thanks.
27 February 2014 376 40 View
I was trying to make a tree/reconstruction with ML and Bayesian inference (BI) trees. I can make them individually but still don't know how to make/reconstruct trees. Could you please explain? Thanks!
12 February 2014 4,022 4 View
I would like to take a DNA sequence from NCBI database with my samples' sequence as an outgroup to make a phylogenetic tree. so in this case, do I need to do alignment and eliminate that sequence...
06 February 2014 8,403 19 View
To be more clear, I have 3 different markers that are all mtDNA (D-loop, cytb and 12s rRNA). I used them for the same fish species and want to analyze them all together. If you have any papers on...
15 December 2013 5,196 1 View
Do we need to edit (i.e. fill the gap, nucleotide edit) sequence data after alignment or not?Needed for further analysis (run with Arlequin, MEGA etc). If needed to edit after alignment (I have...
08 December 2013 7,906 22 View
I am facing problem with PCR purification. I got good PCR band but after purification (by Qiagen purification kit) can't see good band in gel. Can anyone help? I want to sequence my sample.
11 November 2013 7,903 19 View
