Agustina Lino

6 Questions 47 Answers 0 Followers

Questions related from Agustina Lino

Transfection vector for stem cells silencing?

i'm currently looking for what would be the best vector/plasmid for silencing genes in rat stem cells culture. I found some paper where they use lipofectamine, plus reagent, vector pCU and pCH. I...

02 February 2017 8,765 1 View

Why can't i get ladder resolution?

I attach a photo of my agarose gel.. As you can see the DNA ladder runs like "all toghether" (sorry for bad english). I followed the manufacturer recomendations (1,5% agarose, 90mV, 1 hour)... The...

06 June 2016 4,655 11 View

Why didn't PCR work?

I'm doing RT-PCR from rat catilage tissue, and the thing is i searched for tissue specific markers such as SOX9, Aggrecan and type 2 collagen. this is what it looks like, the last 3 lanes are the...

04 April 2016 7,396 10 View

Why do i get a smear on PCR???

i'm doing semiquantitative PCR (RT-PCR reverse transcription) of Alkaline phosphatase from Rat Bone RNA. The primers seq are fw GACAGCAAGCCCAAGAGA rv CAGTTCAGTGCGGTTCCA I attach a picture oh...

01 January 1970 2,519 0 View

BSA-AGE without centricon?

i need to prepare BSA-AGE for my stem cells culture. The problem is i have centricones 100 kDa, i the ones i need are the 10 kDa ones. Is there another protocol for preparing BSA-AGE without...

01 January 1970 9,853 3 View

How can i get rid of bright background on agarose gel??

I'm running my pcr products in an 1% agarose gel made with TAE buffer. The volume i use is 20 ml with 1ul EtBr. I attach a picture. I want to get as dark as posible for the background and as...

01 January 1970 9,079 11 View