Identified my marine bacteria using 16s RNA primer (27F and 1492R)?

I took 20 ul broth culture bacteria and added to 180 ml miliqi water. And boiled 5 minutes, followed by keep in -80oC for 10 minute.To got aliquot of DNA, I centrifuge its for 5 minute. I only use...

01 January 2016 6,583 3 View