RAW 264.7 cells detaching from the plate and appearing not to polarize?

I am seeding 1 x 10(5) or 2 x 10(5) in a 24-well plate with DMEM 10% FBS for two days. Polarizing cells with M0, M1 (1ug/ml LPS + 25 U/ml IFNy) and M2 (40ng/ml IL-4) for 24 hours. I have groups...

13 October 2021 2,417 1 View