Agata Lena Pruska

3 Questions 1 Answers 0 Followers

Questions related from Agata Lena Pruska

How to check for common exons in mRNA isoforms?

How can I check the exon composition of mRNA isoforms in the NCBI database? I am doing a CRISPR Cas9 with guide RNAs designed to target the second exon found in the "majority of the isoforms"...

14 January 2025 1,197 0 View

How to adjust an electrostatic potential surface in pyMol after adding phosphorylation sites?

I used I-TASSER to predict the 3D structure of my protein of interest and PyMOL with APBS plugin to add a surface showing the electrostatic potential. Then, with PyTMs plugin, I added three...

26 January 2024 6,259 0 View

How to optimize a protein structure in PyMol after adding phospho residues?

I am using PyMol with PyTMs plugin to visualize possible phosphorylation sites on a protein. I am wondering how I can use the program to calculate and show an optimized protein structure to avoid...

27 November 2023 7,692 1 View