Agata Michalska

3 Questions 5 Answers 0 Followers

Questions related from Agata Michalska

Additional bands on Western blot after protein overexpression? What are the additional products?

Dear Researchers, I cloned the IRF9 coding sequence into plasmid LV073. I used the Gibson assebly (NEBuilder) protocol. I cut the vector in MCS with the enzyme ScaI (blunt ends), amplified my...

27 November 2020 5,183 4 View

Has anyone pooled libraries from ChIP and RNA experiments in one lane for sequencing?

I have one lane and samples from two experiments (RNA and ChIP). Is there an option to sequence them together? I am afraid that RNA samples will "eat" all the reads. Thank you for any suggestion!

22 July 2020 1,979 4 View

Can I measure expression of MIR genes on Illumina BeadChip if I prepare cRNA using Ambion Total Prep Kit (based on oligodT primers)?

Because list of probes on the BeadChip contains some of MIR genes (i.e. MIR1-2, MIR1200, MIR1202, MIR1203 etc.) however, as I remember correctly - the mature microRNAs don't contain the polyA...

20 June 2016 2,953 3 View