Afshin Samali

2 Questions 14 Answers 0 Followers

Questions related from Afshin Samali

What are the best methods for the detection of macroautophagy?

We have been detecting LC3 lipidation and p62 reduction by western blotting in our studies. However, neither of these features are specific to macroautophagy. These changes do not always...

24 December 2014 10,097 5 View

Are there any cell-based assays to detect the activation of all 3 arms of UPR simultaneously at the single cell level?

I want to detect the kinetics of the activation of IRE1, PERK and ATF6 at single cell level. Ideally cells expressing reporter constructs of equal efficiency to be able to detect temporal...

10 October 2014 5,147 3 View