Afshan Mehreen

2 Questions 1 Answers 0 Followers

Questions related from Afshan Mehreen

Smear band in PCR using primers with added sp6 and T7 promoter?

I have purified DNA of around 150 b.p whose sequence is similar to my gene of interest. Whenever I do normal PCR using common primers I get the band. While I have designed primers to later use for...

4 weeks ago 7,410 5 View

Double bands in purified PCR product. what to do?

I have purified my PCR product but after that I ran the gel and it showed double bands as shown in photo. what should I do. should I sent it for sequencing or not?

13 April 2025 270 4 View