Afsaneh Golkar-Narenji

4 Questions 2 Answers 0 Followers

Questions related from Afsaneh Golkar-Narenji

What is a good protocol for making 12.5% poly acrylamide gel for DNA?

i need a proper protocol to make a good 12.5% poly acrylamid gel for nucleotide (50-36 bp) separation.

08 August 2018 7,928 5 View

Can anyone tell me how I can successfully culture chicken embryonic stem cells?

I am trying to produce chicken embryonic stem cell lines, but after one week they are producing vacuoles, lipids, and finally they are lost. Is it really possible to produce CEs cell line?

04 April 2014 5,260 2 View

Which temprature is the best for keeping spleen tissue frozen before RNA extraction, -70 or liquid nitrogen?

Spleen tissue

01 January 2013 1,714 11 View

How to culture KG1a cells?

How to culture KG1a (nonadherence) cells? KG1a cells seems to be highly sensitive cells and not easily amplified. Does anyone have recommendations for the best culture medium?

07 July 2012 9,694 4 View