Affrida Abu Hassan

4 Questions 8 Answers 0 Followers

Questions related from Affrida Abu Hassan

The difference between cDNA-AFLP and HiCEP?

Both methods use cDNA and digest with RE. Can anyone explain the difference between cDNA-AFLP and HiCEP? TQ

09 September 2015 677 0 View

What are these extra bands in my RNA?

Here is the RNA RIN sheet. The are some extra bands appeared in all my samples. I wonder what these bands are.

08 August 2015 846 4 View

Can I used Tris HCL instead of Tris base to prepare TBE buffer?

As above

07 July 2014 8,160 1 View

How can I determine the concentration of cDNA to be used in restriction ligation step for AFLP?

I am using Invitrogen Core reagent Kit. According to the protocol the amount of DNA/cDNA needed is 100-500ng. The amount of RNA for cDNA synthesis is 2ug in 20ul reaction. Kindly can anyone could...

06 June 2014 8,762 2 View