Adrien Decheix

3 Questions 4 Answers 0 Followers

Questions related from Adrien Decheix

Does anyone have experience with human cell coculture concentration rate?

We have seeded normal human colon fibroblasts (CCD18-co) with human colorectal adenocarcinoma (HT29) within an hyaluronic acid scaffold. We haven't noticed any fibroblasts proliferation in 2D and...

28 May 2015 5,820 0 View

Does anyone have experience with 3D PANC-1 cell culture? peripheral tubulin labelled by immunofluorescence in particular?

We have seeded PANC-1 within HA scaffold and we have noticed a peripheral tubulin labelled (spheroid picture attached). What is your opinion?

19 May 2015 345 5 View

Does anyone have experience with 3D keratinoytes cell culture (HPV16 oncogenes E6 and E7)?

We have seeded these cell line within HA hydrogel and since the keratinocytes have formed spheroids overnight (50000 per well) we were thinking that it’s unlikely these spheroids are generated...

12 May 2015 6,299 3 View