Adrian Kaczmarek

5 Questions 32 Answers 0 Followers

Questions related from Adrian Kaczmarek

Can someone offer an explanation for two methods of Immunoblotting phosphorylated proteins?

I'm looking to detect pFAK Tyr397. I've found two methods, the first is to run whole lysates on a gel, and blot for a pFAK antibody (ie pFAK-397) and the other is to IP the FAK out of the lysate,...

11 November 2015 8,875 5 View

Culture and Proliferation of BMDM (Bone Marrow Derived Macrophages)

I was wondering if anyone can describe their experience with BMDM culture and propagation (growing up more cells). I have a We have isolated BMDM from the Femurs of Balb/C mice by seeding Marrow...

07 July 2015 9,309 24 View

Has anyone experienced an inability for protein specific antibodies to bind their target when the protein has a Flag Tag?

That is, an antibody, which binds a protein on a western blot very specifically, appears not to bind at all when the protein has a FLAG or 3xFLAG tag?

07 July 2013 7,601 3 View

Can all mouse cell lines express proteins within a vector driven by a human CMV promoter?

I have created a stable transfection of a mouse mammary cell line 4T1. However my gene of interest is not being expressed in the 4T1 cell line. On the other hand it is expressed strongly in 293...

06 June 2013 6,527 19 View

Freestyle and other serum free media?

Has anyone grown adherent cell lines (CHO-K and 293) in the Freestyle range of media's? Or another type of serum free media optimised for protein expression and purification? Traditionally these...

06 June 2013 1,033 6 View