Adrian Dario Juncos Bombin

3 Questions 2 Answers 0 Followers

Questions related from Adrian Dario Juncos Bombin

How much time incubate a transfection assay with single-stranded RNA?

Hi all, I have nanoparticles that encapsulate and deliver to cells a ssRNA of 21 nucleotides (that is supposed to act as a microRNA). I am going to transfect those nanoparticles into NCTC 929...

01 January 2019 4,842 5 View

What factors to consider when designing artificial microRNA?

Hello, I am planning to design a ssRNA molecule that I will use as a microRNA to block the translation of GFP. I will use this as a positive control for transfection. My question is, what factors...

11 November 2018 6,514 3 View

Is Enhanced GFP post-transcriptionally modified?

Dear all, I want to design a microRNA that targets EGFP to downregulate its expression. I find that the actual DNA sequence of EGFP codes for a Valine that would be the second amino acid, but...

11 November 2018 5,666 3 View