Adrian Kaczmarek

3 Questions 15 Answers 0 Followers

Questions related from Adrian Kaczmarek

Can someone help with a migration assay using RAW264.7 cells?

I am trying to use RAW264.7 macrophages to observe migration. The ultimate aim is to treat these cells with compounds and see how they migrate. At first however I wanted to just establish the...

03 April 2015 4,331 9 View

What are the classical actin-structural features of a migrating cell?

Hi all, I'm a novice in this field of cellular actin restructuring... I am treating RAW264.7 macrophages with a chemokine and hoping to demonstrate actin reorganization of the cell during...

24 March 2015 8,092 6 View

Can all mouse cell lines express proteins within a vector driven by a human CMV promoter?

I have created a stable transfection of a mouse mammary cell line 4T1. However my gene of interest is not being expressed in the 4T1 cell line. On the other hand it is expressed strongly in 293...

16 June 2013 7,088 18 View