Adrian Dario Juncos Bombin

4 Questions 2 Answers 0 Followers

Questions related from Adrian Dario Juncos Bombin

How do you calculate the standard error of the mean when you repeat an experiment three times and take three samples out of each repeat (N=3, n=3)?

Hi all, Let's assume you want to do an experiment. For the results to be as accurate as possible, you repeat this experiment three times keeping all variables exactly the same (N=3). And each...

28 April 2020 5,968 1 View

How much time incubate a transfection assay with single-stranded RNA?

Hi all, I have nanoparticles that encapsulate and deliver to cells a ssRNA of 21 nucleotides (that is supposed to act as a microRNA). I am going to transfect those nanoparticles into NCTC 929...

31 January 2019 779 4 View

What factors to consider when designing artificial microRNA?

Hello, I am planning to design a ssRNA molecule that I will use as a microRNA to block the translation of GFP. I will use this as a positive control for transfection. My question is, what factors...

13 November 2018 5,625 3 View

Is Enhanced GFP post-transcriptionally modified?

Dear all, I want to design a microRNA that targets EGFP to downregulate its expression. I find that the actual DNA sequence of EGFP codes for a Valine that would be the second amino acid, but...

08 November 2018 7,613 3 View