Aditya Dandekar

3 Questions 9 Answers 0 Followers

Questions related from Aditya Dandekar

Detecting a ubiquitinated protein in vivo?

I am looking at ubiquitination of protein caused by inflammation. When I do my experiments in HEK293 cells with overexpression of my tagged protein, I see typical ubiquitin smear for pulldown...

11 November 2013 4,049 2 View

Good model cell line for PI3K-Akt signaling.

I am looking for a cell line which doesn't show constitutive activation of the PI3K-Akt pathway. I am planning to do a protein interaction study using activators of this pathway like Insulin,...

02 February 2013 6,657 4 View

Why does semi-quantitative PCR at 30 and 35 cycles produce different results?

I have been doing semi-quantitiative PCR for detecting TLR expression in mouse primary hepatocytes. I am facing a problem with my current set of primers. After optimization, I am getting no band...

11 November 2012 1,582 16 View