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Hello everyone, for invitro cleavage of recombinant protein, i need to use Enterokinase. Protein is getting cleaved properly, but i think its being degraded as well. Anyone ever used Protease...
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hello all, I have transformed a 20kb plasmid into E.coli JM109. But the problem is the growth time has become too high, may be more than 30 hrs and so. Colonies start appearing on plants after...
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Hello, I am trying to purify a recombinant protein (~15kD) from Tobacco. I am using Agroinfiltration based expression system. Expression is high, but when it comes to purification, I could not get...
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Hello everyone, I am trying to clone TGFB1 gene into my expression cassette. But when I am doing ligation followed by transformation, I get colonies on Vector Control plate (might be due to...
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Hello Everyone, I am trying to develop a protein expression and purification system. Hence, can anyone tell me about "Intein Tags" or related papers, articles or suggestion? Please Help Thanks
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hello all, I am trying to produce His-Human-TGFB1 in tobacco leaf tissues using agrobacterium mediated transient expression system. The Purification is based on His-Tag Ni-NTA system. Protein is...
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Hello All, I am having an issue that is conceptually still very confusing for all those who know about the Geminiviral based protein expression vector used to produce recombinant proteins in...
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