Aditya Soni

3 Questions 8 Answers 0 Followers

Questions related from Aditya Soni

Any recommended method to grow E.coli JM109 having a plasmid size of 20kb?

hello all, I have transformed a 20kb plasmid into E.coli JM109. But the problem is the growth time has become too high, may be more than 30 hrs and so. Colonies start appearing on plants after...

23 July 2018 8,800 5 View

"Intein Protein Tag", someone Knows?

Hello Everyone, I am trying to develop a protein expression and purification system. Hence, can anyone tell me about "Intein Tags" or related papers, articles or suggestion? Please Help Thanks

09 January 2017 3,174 3 View

His-Tagged Human TGFB1 purification from Nicotiana benthamiana

hello all, I am trying to produce His-Human-TGFB1 in tobacco leaf tissues using agrobacterium mediated transient expression system. The Purification is based on His-Tag Ni-NTA system. Protein is...

01 January 1970 432 2 View