Aditya Misra

6 Questions 9 Answers 0 Followers

Questions related from Aditya Misra

How to add inlets to PDMS fluidics device?

I'm designing a PDMS fluidics device that has millimeter-wide channels (~1-2mm wide), and I'd like to put inlets on it. The PDMS layer is 5-6 mm thick and is plasma bonded to glass. So far, I've...

25 July 2021 8,836 3 View

Creating an infection reporter plasmid that is transcribed/translated by mammalian cells?

Hi, I'd like to create a plasmid that a pathogen (like Klebsiella pneumoniae) can carry/replicate into the mammalian cells it infects. Then, that plasmid (assuming a small number of them survive...

18 July 2021 4,459 3 View

How to design homology arms for HDR template to N-terminal tag a gene of interest?

I am trying to tag moxCerulean3 to the N-terminus of a gene of interest. In other words, I'd like to place this reporter on the first exon of the gene right after the start codon. I've been using...

10 February 2020 7,863 1 View

Can precipitate occur in chemically defined media used to grow E. coli?

I am trying to grow up E. coli in a chemically defined media. Currently, I am using a recipe from a paper released by Eppendorf as a guide. In my recipe, I have - 100 mL of citric acid/phosphate...

16 March 2015 5,979 7 View

What is the best way to carry out differential scanning calorimetry for bacterial membranes?

I would like to study the freezing and melting of bacterial membranes and would like to use DSC to obtain the glass transition temperature of the membrane. However, I am unsure where to find such...

11 August 2014 9,285 7 View

How can I carry out scale up of E.Coli culture?

I am currently growing up E.Coli in 40mL of growth medium (LB) in a 250mL shaker flask... I would like to scale up this process to a 1-5L large scale setup for the lab. But for now I'd like to...

25 July 2014 5,839 6 View