Aditya Iyer

9 Questions 22 Answers 0 Followers

Questions related from Aditya Iyer

What is the trigger to open the Aquaporin channel in E.coli under osmotic stress ?

I am digging into the trigger that opens the AquaporinZ (aqpZ) water channel in E.coli. Although most papers suggest involvement of residues Arginine-189 and the F43, H174 and T183, I did not...

14 October 2020 7,248 0 View

Expresion of a periplasmic protein ?

I am interesting in purifying a periplasmic protein from E.coli. It is not membrane bound. Is there is book/review I can refer to optmise/maximise protein production.?

14 March 2017 2,068 4 View

Influence of delocalized electrons in Arginine?

A recent discussion amongst colleagues brought this question up and I hope some of you can help me here. We have been taught for a long time that Arginine as an amino acid is positively charged. A...

13 September 2016 7,776 1 View

Are Self-quenching and homo-FRET in fluorophores physically different processes?

I know homo-FRET requires distances of the order of 3-8 nm while self-quenching requires a much closer contact (~ 1 nm). But the distinction between the two processes isn't very clear..Any...

28 March 2016 3,865 4 View

Procuring TMV rods for Electron Microscopy?

Do any of you guys know how one could get access to some Tobacco Mosaic Virus rods for Electron microscopy? Apparently they are not available commercially or I haven't come across any suppliers?...

24 February 2016 1,306 1 View

Chloride absorbance in CD spectra?

I have a question about chloride absorbance in CD spectra of proteins. It is well known that Cl- ions absorb strongly at wavelength less than 195 nm. Suppose I take a solution of 100 mM NaCl and...

19 November 2015 6,698 9 View

Is there a script/plugin for bulk analyzing periodicities in amyloid fibrils?

I want to know if there ia a script/plugin for analyzing periodicities in amyloid fibrils in a batch mode. Analysing periodicities in individual images is a tedious task and I was wondering if...

02 July 2014 7,464 9 View

Labeling animal cells with fluorescent lipids?

I wish to label SHS5Y cells with a fluorescent lipid dye so that I can measure the diffusion coefficient of the dye in a plasma membrane. I have to use Rhodamine-DOPE or another red dye. Does...

19 March 2013 2,074 11 View

Diffusion Coefficient of NBD-PC on supported lipid bilayers

I have been working on supported lipid bilayers on glass slides for sometime now. I use pure POPC (neutral lipid) for my experiments. The issue is that the diffusion coefficients i get is less...

04 October 2012 5,544 3 View