Aditya Dandekar

2 Questions 5 Answers 0 Followers

Questions related from Aditya Dandekar

Detecting a ubiquitinated protein in vivo?

I am looking at ubiquitination of protein caused by inflammation. When I do my experiments in HEK293 cells with overexpression of my tagged protein, I see typical ubiquitin smear for pulldown...

25 November 2013 5,623 2 View

Why does semi-quantitative PCR at 30 and 35 cycles produce different results?

I have been doing semi-quantitiative PCR for detecting TLR expression in mouse primary hepatocytes. I am facing a problem with my current set of primers. After optimization, I am getting no band...

02 November 2012 143 15 View