Aditya Chandru

2 Questions 1 Answers 0 Followers

Questions related from Aditya Chandru

Ribosome profiling. Trying to specifically get only monosomes and (primarily) disomes from a sucrose gradient. What range of sucrose do I use? 10-30%?

Running a sucrose gradient and I am only interested in the disome peak, would also like to separate out the monosome peak. Not interested in polysomes past this. We usually run a gradient 10-50%...

16 May 2022 7,293 1 View

Anyone have a protocol for using N-Ethylmaleimide (deSUMO inhibitor) in a nuclear extraction?

Does anyone have any experience with NEM (N-Ethylmaleimide) in a nuclear extraction? I am trying to stabilize the SUMO modifications of a protein during my nuclear extraction for a western...

13 November 2020 8,910 1 View