Bumpy melting curves in qrtpcr? I am having some extra picks in my melting curves. I think is primer dimer since it is in NTC?

I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...

06 November 2017 5,994 7 View